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柑橘副產(chǎn)物發(fā)酵益生菌作為膳食補充劑對于牙鲆幼魚生長性能、非特異性免疫和抗病性的效果

《魚類疾病月刊》2013, 36, 617–628  

摘要

本實驗進行了兩項連續(xù)研究，評估的柑橘益生菌發(fā)酵副產(chǎn)品(CBF) 作為膳食補充劑對于牙鲆幼魚生長性能、飼料利用率、非特異性免疫和抗病性的效果。實驗1，牙鲆幼魚共分為5組，分別是空白組（CON，飼料中不添加CBF），CBF-BS（飼料中添加3%枯草芽孢桿菌發(fā)酵產(chǎn)物），CBF-EF（飼料中添加3%屎腸球菌發(fā)酵產(chǎn)物），CBF-LR（飼料中添加3%鼠李糖乳桿菌發(fā)酵產(chǎn)物）和CBF-LP（飼料中添加3%植物乳酸桿菌發(fā)酵產(chǎn)物）。，十周飼喂結(jié)束進行測定。實驗結(jié)果表明，各組之間生長性能和飼料利用率差異不顯著，但是飼喂CBF的牙鲆幼魚存活率顯著高于空白組，且對于遲鈍愛德華氏菌的抵抗性能明顯提高。在實驗2中，我們選擇CBF-BS進行進一步分析。牙鲆幼魚分別飼喂含添加0%，2%，4%，6%和8%的飼枯草芽孢桿菌發(fā)酵產(chǎn)物。6周飼喂實驗結(jié)束進行測定。實驗結(jié)果表明，CBF-BS對于牙鲆幼魚生長性能影響不顯著，但是可以有效提高幼魚的非特異免疫力，幼魚溶菌酶和髓過氧化物酶活性呈現(xiàn)劑量依賴性的增加。針對遲鈍愛德華氏菌的連續(xù)性激發(fā)試驗表明，CBF-BS能有效增加幼魚的抗病能力。

Effects of dietary supplementation of citrus by-products fermented with a probiotic microbe on growth performance, innate immunity and disease resistance against Edwardsiella tarda in juvenile olive flounder, Paralichthys olivaceus (Temminck & Schlegel) 

B-J Lee, S-S Kim, J-W Song, D-H Oh, J-H Cha, J-B Jeong, M-S Heo, K-W Kim and K-J Lee.

 Journal of Fish Diseases, 2013, 36, 617–628 

Abstract

Two consecutive studies were conducted to evaluate the dietary supplementation of citrus by-products (CB) fermented with probiotic bacteria on growth performance, feed utilization, innate immune responses and disease resistance of juvenile olive flounder. In Experiment I, five diets were formulated to contain 0% (control) or 3% four different CB fermented with Bacillus subtilis(BS), Enterococcus faecium (EF), Lactobacillus rhamnosus(LR) and L. plantarum (LP) (designated as CON, CBF-BS, CBF-EF, CBF-LR and CBF-LP, respectively). During 10 weeks of a feeding trial, growth performance and feed efficiency were not significantly different among all the fish groups. However, fish fed CBF containing diets had significantly higher survivals than the CON group. Disease resistance of fish against Edwardsiella tarda was increased by the fermentation of CB. In Experiment II, we chose the BS as a promising probiotic and formulated five diets to contain 0%, 2%, 4%, 6% and 8% CBF-BS. Growth performance was not significantly affected by the CBF-BS supplementation during 6 weeks of a feeding trial. Innate immunity of fish was significantly enhanced by CBF-BS supplementation. Myeloperoxidase and lysozyme activities were increased in a dose-dependent manner by dietary CBF-BS inclusions. In a consecutive challenge test against E. tarda, an increased disease resistance was found by CBF-BS supplementation. These studies indicate that the fermentation process of CB with probiotic has beneficial effects on innate immunity and thereby increases disease resistance of olive flounder

against E. tarda. Bacillus subtiliscan be used as a promising probiotic microbe for by-product fermentation in fish feeds.